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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two supplemental splice variants were described but in depth nucleotide sequence information is not still available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This guideline outlines further possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Information Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and it is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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